Table 1.
The sequences used in cloning the cps-1 gene and for cloning the upstream regulatory region are provided. The SpeI, NotI and XmaI sites used for cloning the cps-1 gene are all inside the amplified DNA sequences.
Primer name | Sequence (5’ to 3’) |
---|---|
For cloning of cps-1 | |
cps-1 5’ end forward primer | ATTAACTAGTCGGAGTCATCTGTCAATGCAG |
cps-1 3’ end reverse primer | AAATGCGGCCGCCCAATCTCCTCATGCCTCTGC |
For cps-1 RIP experiment | |
cps-1 RIP forward primer | ATTACCCGGGACAAGGTTGCCCTTTCCTTC |
cps-1 RIP reverse primer | GCACTAGTCAAGCATACCATATAACAATATGG |
For cloning the HA-cps-1 | |
HA-cps-1 forward primer | TACCCCTACGACGTCCCCGACTACGCCTAACGG TACTGGCTTGCTGCT |
HA-cps-1 reverse primer | GGCGTAGTCGGGGACGTCGTAGGGGTAACCTTG CAGAGATGGGGGTGA |