Table 2.
Analysis of synergism of Cyt1Aa or mutant toxins with Cry11Aa toxin.
| Toxin | S(toxin)OBSa = (Rep1 + Rep2 + Rep3)/n |
S(Cyt1Aa, Cry11Aa)EXPb = S(Cyt1Aa)OBS × S(Cry11Aa)OBS |
Expected mortalityc = (1 − S(Cyt1Aa, Cry11Aa)EXP) × 100% |
Observed mortalitydCyt1Aa + Cry11Aa |
|---|---|---|---|---|
| Cyt1Aa | 1.00 | 0.80 | 20% | 90 ± 10% |
| L120K | 1.00 | 0.80 | 20% | 93 ± 6% |
| V122E | 1.00 | 0.80 | 20% | 57 ± 15 % |
| L123K | 1.00 | 0.80 | 20% | 90 ± 10% |
| V126E | 1.00 | 0.80 | 20% | 77 ± 15% |
| Cry11Aa | 0.80 |
Observed survival of individual toxin S(toxin)OBS corresponds to the observed proportion of larvae that survived to the exposure to Cyt1Aa or mutant toxins. Observed mortality was 20% with Cry11Aa at 200 ng ml−1 and 0% with Cyt1Aa or mutant toxins at 75 ng per ml. n = 30 larvae for each toxin tested.
Theoretical proportion of larvae that survive to the toxin-mixture, S(Cyt1Aa, Cry1Aa)EXP = S(Cyt1Aa)OBS × S(Cry11Aa)OBS corresponds to the proportion of larvae expected to survive to the exposure of a mixture of toxins.
Theoretical expected mortality was calculated with (1−S(Cyt1Aa, Cry11Aa)EXP) × 100%.
Experimentally observed mortality with the mixture of toxins using Cry11Aa at 200 ng ml−1 of plus each Cyt1Aa toxin at 75 ng ml−1. These assays were performed three times. Fisher’s exact test showed values of P < 0.001 for each comparison.