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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Exp Cell Res. 2014 Apr 25;326(2):295–306. doi: 10.1016/j.yexcr.2014.04.013

Figure 3. Differential effects of blebbistatin on fibroblast contraction of stressed and floating collagen matrices.

Figure 3

Stressed collagen matrix contraction was carried out in DMEM/FBS. Floating collagen matrix contraction was carried out in DMEM/PDGF and DMEM/FBS. Blebbistatin as indicated was added to the incubations at the times that the matrices were released from the underlying culture dishes to initiate contraction -- 18 hr for SMC and 1 hr for FMC. Data shown are the appearance of collagen matrices from a representative experiment and the averages ± SD of quantitative contraction measurements (starting – final matrix diameter) for triplicate samples. Blebbistatin inhibited SMC-FBS and FMC-PDGF at 2.5 μM, but 10-20 μM blebbistatin was required to inhibit FBS-FMC.