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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Exp Cell Res. 2014 Apr 25;326(2):295–306. doi: 10.1016/j.yexcr.2014.04.013

Figure 4. Differential effects of silencing MyoIIA and IIB on floating and stressed collagen matrix contraction.

Figure 4

A,B) Stressed matrix contraction was carried out with mock and myosin II isoform-silenced cells as indicated and untreated cells plus 20 μM blebbistatin (bleb) in DMEM/BSA, DMEM/FBS, and DMEM/PDGF as shown. C,D). Floating collagen matrix contraction was carried out with mock and myosin II isoform-silenced cells as indicated in DMEM/BSA, DMEM/FBS, and DMEM/PDGF as shown. Data presented are the averages ± SD of quantitative contraction measurements (starting – final matrix diameter) for triplicate samples. Complete set of p values are shown in supplemental Table 1. Silencing MyoIIA inhibited SMC as well as addition of 20 μM blebbistatin, whereas silencing MyoIIB did not have a detectable effect. Silencing either MyoIIA or MyoIIB separately had no effect on FMC; silencing both together inhibited PDGF-FMC but not FBS-FMC.