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. Author manuscript; available in PMC: 2014 Jul 29.
Published in final edited form as: Int J Cancer. 2013 Feb 8;132(11):2578–2588. doi: 10.1002/ijc.27936

Figure 1.

Figure 1

Cell-SELEX scheme and progression. (a) Flow cytometry dot-plot of E-cadherin-PE staining versus forward scatter signal of DU145 cell line. Gates that select for E-cad+ and E-cad− are shown as polygons (left panel). Unsorted cells are green, E-cad+ are blue, and E-cad− are red. The purity of E-cad+ (middle panel) and E-cad− (right panel) sorted cells was confirmed by post-sorting flow cytometry as shown in the middle and right panels, respectively. (b) Flow cytometry histograms of enriched pools from selection rounds 14 (red), 16, 18 (blue), 19 (green) and 20 (yellow) binding to E-cad+ and E-cad− cells of DU145. A significant increase in intensity is observed for all selection rounds above 15 for E-cad+ cells compared E-cad− cells. The directions of the arrows show increase in fluorescence intensity as selection progressed. (c) DU145 cells positive and negative for the 20th and 24th selection round of enriched pools and unsorted DU145 cells were plated at the same density and allowed to grow for 3 days. The cells positive for the 20th and 24th selection rounds of enriched pools showed higher proliferative capacity compared to negative and unsorted cells. P: positive cells, N: negative cells, C: Control cells. *, P < 0.05 in comparison to negative cells Representative results of two independent experiments are shown. (d) Representaive phase contrast image of cell growth for positive and negative subpopulation for the 24th selection round. Bar = 100 μm.