Skip to main content
. Author manuscript; available in PMC: 2014 Nov 29.
Published in final edited form as: Nat Commun. 2014 May 29;5:3923. doi: 10.1038/ncomms4923

Figure 5. BADJ formation is independent of branch size and generation.

Figure 5

(a, b) OPT images of whole mount immunostained lungs from Sftpc-rtTA; TetO-DTA (treated with doxycycline from E13 to E14) or Igf1r−/− mutant and their respective littermate control embryos. The L.L6 branch lineages 14 in the boxed areas are enlarged and traced with dashed lines in subsequent images. At least two experiments for each time point are performed with similar results. As illustrated in the diagram (c), in the Sftpc-rtTA; TetO-DTA and Igf1r−/− mutant lungs, the first wave is still marked by SOX9 expression but progresses much slower than that in the control lungs; and the second wave follows the first wave early in development and terminates on schedule at E17.