Figure 4.
Delayed myelin repair in LPC-lesioned spinal cord of Cdk5 cKO mice. A, Deletion of Cdk5 in CNP+ cells and marked with YFP reporter to define the Cre recombination rate. Similar ∼70% recombination rate showed in CC1+ cells (red) were double-labeled with YFP (GFP, green) in both CNPcre/+;Cdk5+/+; RosaYFP/+ WT (Aa) and CNPcre/+;Cdk5fl/fl; RosaYFP cKO (Ab) animals. Ac, Western blot analyses. B, Luxol fast blue staining showed larger lesioned spinal cord volumes in Cdk5 cKO mice (Bb, Bd, Bf, Bh) compared with WT mice (Ba, Bc, Be, Bg) at 3, 7, 14, and 21 dpl. C, D, Quantitative analysis of the volume of lesioned spinal cord in Cdk5 cKO mice and WT mice (C), and the progress of remyelination at 3, 7, 14, and 21 dpl. Values are mean ± SEM (n = 3). *p < 0.05. Scale bar, 25 μm.