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. 2014 Jul 19;21(1):64. doi: 10.1186/s12929-014-0064-4

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Copyright © 2014 Chou et al.; licensee BioMed Central

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Isolation ofDadi1 alleles. (A) Schematic diagram of the methionine salvage and recycling pathways, which is generated based on metabolism pathways in the Kyoto Encyclopedia Genes and Genomes (KEGG) database. The Main enzyme studied here is highlighted with yellow. (B) A map of genomic DNA displays the Dadi1 (CG32068) locus. The DADI1^d01129 P-element insertion line (black arrow) contains a p-element at 48 bp upstream of the DADI1 start codon (red arrow). Three DADI1 mutant alleles (Dadi1⁷, Dadi1⁹ and Dadi1⁷⁴) were produced with alterations in the Dadi1 coding region. (C) Immunoblotting of DADI1 in several developmental stages. Embryos were collected after egg deposition. (D) The protein levels of DADI1 were reduced in Dadi1⁷⁴ heterozygote strain (Dadi1⁷⁴/TM3Ser). Western blot analysis of DADI1 protein levels of Dadi1⁷/ Dadi1⁹ trans-heterozygous alleles and Dadi1⁷⁴ homozygous mutant indicated that they were protein null alleles.