Figure 3.

Enzymatic activity of ADI1 is essential for regulating egg production. (A) Protein sequence alignment is shown in the Cupin domain of hADI1 and DADI1. The identity and similarity of Cupin domain is 68% and 84%, respectively. The glutamic acid site is required the ARD/ARD’ family to execute enzymatic function (red star). (B) The protein expression levels of human wild-type and E94A mutant transgenic lines were observed in the Dadi1 null mutant background by hADI1 antibody. (C) Expressing human ADI1 by Actin-Gal4 can rescue the egg production defect in Dadi1 mutant alleles. (D) The fecundity was not rescued by diverse expressing enzyme dead mutant (E94A) transgenic lines. *P < 0.05, **P < 0.01 and N.S., no significance.