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. 2014 Jul 17;10(7):798–806. doi: 10.7150/ijbs.9470

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The level of Lcn2 in rat PH model induced with MCT. 7, 14, 21, 28 and 35 days after the subcutaneous injection of MCT or saline, the lung samples of rats were collected. A, Total protein was extracted and subjected to Western blotting analyses. Representative western blot and quantitative analysis are shown (n = 6; *, p < 0.05 compared with control). B, Total RNA was extracted and Real-time PCR for Lcn2 was performed (n = 6; *, p < 0.05 compared with control). C, Immunostaining for LCN2 in lungs of rat after 3 weeks of MCT (or saline) injection (400×).