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Lcn2 decreases Bax expression. HPASMCs were treated with serum free medium or 100 μM H2O2, in the presence or absence of 10 ng/ml Lcn2 for 24 h. A, Total RNA was extracted and reverse transcripted to cDNA, quantitative determination of Bax expression using real-time PCR, and normalized against the internal standard GAPDH. B, Total protein was extracted and subjected to Western blotting analyses. A representative blot and quantitative analysis are shown. (n = 3; *, p < 0.05).
