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Inactivation of miR-146a expression by ASO, led to reduced expression of miR-146a as assessed by qRT-PCR (A), increased cell migration by scratch assay (B), increased EGFR expression by western blot analysis (C) and increased in colony formation by clonogenic assay (D). These changes were overcome by CDF treatment. RNU48 miRNA was used to normalize miR-146a expression. Beta-actin was used as loading control for the western blot.
