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. 2014 Jun 26;12:37. doi: 10.1186/1477-5956-12-37

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Copyright © 2014 Vale et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Protein staining with Coomassie brilliant blue R-250 (CBB) in embryogenic cultures of C. papaya after 42 days of incubation in the control (without PEG) and PEG6 (with 6% PEG) maturation treatments. (A-B) Control treatment; (C-D) PEG6 treatment; (E) protein granules in embryogenic cells; (F) cotyledonary somatic embryo under PEG6 treatment. Red arrow: non-embryogenic cells; black arrows: embryogenic cells, ma: meristematic aggregates; Asterisks (*): proembryo; pt: protoderm; pr: procambium. PEG: polyethylene glycol. Bars: 200 μm.