. 2014 Jun 26;12:37. doi: 10.1186/1477-5956-12-37

Table 2.

Expressed proteins identified via mass spectrometry in the embryogenic cultures of the C. papaya after 42 days in maturation treatments

Spot^a	Tratament^b	Function^c	Accession number^d	Protein name	Organism	Peptides number^e	Coverage (%)^f	Score^g	Abundance^h
Spot^a	Tratament^b	Function^c	Accession number^d	Protein name	Organism	Peptides number^e	Coverage (%)^f	Score^g	Control PEG6
Proteins of higher abundance in PEG6 treatment
017	Control/PEG6	Stress response, glycolysis	gi\|3914394	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	Mesembryanthemum crystallinum	4	8%	196
021	Control/PEG6	Carbohydrate and energy metabolism	gi\|3023685	Full = Enolase	Alnus glutinosa	3	10%	199
114	Control/PEG6	Stress response	gi\|211906436	UDP-D-glucose pyrophosphorylase	Gossypium hirsutum	4	14%	363
128	PEG6	Stress response, Others functions	gi\|151347486	Methionine synthase	Carica papaya	3	5%	70
130	PEG6	Metabolic process	gi\|1743354	Aldehyde dehydrogenase (NAD+)	Nicotiana tabacum	2	4%	137
138	PEG6	Stress response, oxidation-redution process, Carbohydrate and energy metabolism	gi\|15222848	Glyceraldehyde 3-phosphate dehydrogenase	Arabidopsis thaliana	3	15%	147
139	PEG6	Protein metabolism, stress response	gi\|3914394	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	Mesembryanthemum crystallinum	2	3%	68
142	PEG6	Protein metabolism, stress response	gi\|21487	Leucine aminopeptidase	Solanum tuberosum	1	2%	56
146	PEG6	Oxidation-redution process	gi\|29501684	Alcohol dehydrogenase 3	Petunia x hybrida	3	10%	136
162	PEG6	Protein metabolism, stress response	gi\|38154482	Molecular chaperone Hsp90-1	Nicotiana benthamiana	5	8%	189
163	PEG6	Others functions	gi\|1707372	Ubiquitin-like protein	Arabidopsis thaliana	3	25%	119
165	PEG6	Unclassified	gi\|20140866	Full = Translationally controlled tumor protein homolog	Cucumis melo	2	13%	83
166	PEG6	Other functions	gi\|295885749	Cytokinin oxidase 2	Triticum aestivum	1	1%	46
169	PEG6	Fatty acid metabolism, Carbohydrate and energy metabolism	gi\|136057	Full = Triosephosphate isomerase, cytosolic	Coptis japonica	1	3%	55
175	PEG6	Fatty acid metabolism	gi\|147784332	Hypothetical protein VITISV_041523	Vitis vinifera	2	9%	69
184	PEG6	Metabolic process	gi\|255565327	Esterase D, putative	Ricinus communis	1	6%	46
188	PEG6	Carbohydrate and energy metabolism	gi\|82621108	Phosphoglycerate kinase-like	Solanum tuberosum	2	8%	212
194	PEG6	Oxidation-reduction process, fatty acid metabolism	gi\|2204087	Enoyl-ACP reductase	Arabidopsis thaliana	2	12%	75
200	PEG6	Seed germination, cytoskeleton organization	gi\|32186896	Actin	Gossypium hirsutum	6	22%	454
215	PEG6	Oxidation-redution process, stress response	gi\|449458315	PREDICTED: glutamate dehydrogenase 2-like	Cucumis sativus	2	6%	95
222	PEG6	Others functions	gi\|38564733	Initiation factor eIF4A-15	Helianthus annuus	3	14%	190
227	PEG6	Carbohydrate and energy metabolism	gi\|3023685	Full = Enolase	Alnus glutinosa	2	8%	110
237	PEG6	Stress response	gi\|169661	S-adenosylhomocysteine hydrolase	Petroselinum crispum	1	8%	45
243	PEG6	Carbohydrate and energy metabolism	gi\|3023685	Full = Enolase	Alnus glutinosa	2	8%	130
244	PEG6	Carbohydrate and energy metabolism	gi\|3023685	Full = Enolase	Alnus glutinosa	2	8%	110
250	PEG6	Unclassified	gi\|462413398	Hypothetical protein PRUPE_ppa003850mg	Prunus persica	3	8%	130
Proteins of higher abundance in Control treatment
002	Control/PEG6	Unclassified	gi\|384249809	HI0933-like protein	Coccomyxa subellipsoidea	1	2%	50
011	Control/PEG6	Fatty acid metabolism	gi\|257096376	Full = GDSL esterase/lipase	Carica papaya	2	6%	82
012	Control/PEG6	Fatty acid metabolism	gi\|257096376	Full = GDSL esterase/lipase; AltName: Full = CpEST	Carica papaya	2	6%	82
018	Control/PEG6	Stress response, Carbohydrate and energy metabolism	gi\|414866626	TPA: hypothetical protein ZEAMMB73_999129	Zea mays	2	6%	64
025	Control/PEG6	Oxidation-redution process	gi\|255555109	Flavoprotein wrbA, putative	Ricinus communis	1	11%	112
026	Control/PEG6	Other functions	gi\|162464222	Small GTP binding protein1	Zea mays	2	11%	95
042	Control/PEG6	Carbohydrate and energy metabolism	gi\|14423688	Full = Enolase 1	Hevea brasiliensis	2	8%	62
043	Control/PEG6	Stress response	gi\|2853219	Glutathione transferase	Carica papaya	5	22%	330
048	Control/PEG6	Others functions	gi\|89212810	14-3-3-like protein	Gossypium hirsutum	4	15%	213
049	Control/PEG6	Protein metabolism	gi\|399942	Full = Stromal 70 kDa heat shock-related protein	Pisum sativum	4	9%	205
055	Control/PEG6	Other functions	gi\|162464222	Small GTP binding protein1	Zea mays	2	11%	95
054	Control/PEG6	Stress response, protein metabolism	gi\|475610277	Aspartic proteinase oryzasin-1	Aegilops tauschii	1	2%	60
111	Control/PEG6	Fatty acid metabolism	gi\|257096376	Full = GDSL esterase/lipase	Carica papaya	6	18%	389
115	Control/PEG6	Stress response, Carbohydrate and energy metabolism	gi\|7417426	UDP-glucose pyrophosphorylase	Oryza sativa Indica Group	2	6%	53
315	Control	Stress response, metabolic process	gi\|32527831	UDP-glucose pyrophosphorylase	Populus tremula x Populus tremuloides	3	9%	122
317	Control	Other functions	gi\|225442221	Initiation factor eIF4A-15	Vitis vinifera	4	16%	154
318	Control	Stress response	gi\|470129411	PREDICTED: vicilin-like antimicrobial peptides 2-2-like	Fragaria vesca subsp. Vesca	1	1%	49
324	Control	Protein folding	gi\|166770	Heat shock protein 83	Arabidopsis thaliana	1	2%	66
325	Control	Protein folding	gi\|20559	hsp70 (AA 6–651)	Petunia x hybrid	2	5%	68
326	Control	Protein folding	gi\|20559	hsp70 (AA 6–651)	Petunia x hybrid	2	3%	54
329	Control	Stress response	gi\|226973436	Beta-thioglucoside glucohydrolase	Carica papaya	3	9%	165
333	Control	Oxidation-redution process	gi\|295367043	Cinnamoyl alcohol dehydrogenase	Bambusa multiplex	5	23%	48
335	Control	Carbohydrate and enregy metabolism	gi\|359483362	PREDICTED: lactoylglutathione lyase	Vitis vinifera	3	11%	146
337	Control	Protein folding	gi\|62433284	BiP	Glycine Max	2	5%	75
339	Control	Stress response	gi\|226973436	Beta-thioglucoside glucohydrolase	Prunus pérsica	3	6%	126
340	Control	Stress response	gi\|226973436	Beta-thioglucoside glucohydrolase	Carica papaya	3	6%	108
343	Control	Fatty acid metabolism	gi\|257096376	Full = GDSL esterase/lípase	Carica papaya	2	6%	163
345	Control	Carbohydrate and energy metabolism	gi\|356562473	PREDICTED: xylose isomerase-like	Glycine Max	3	9%	56
347	Control	Other functions	gi\|357440579	Pentatricopeptide repeat-containing protein	Medicago truncatula	7	12%	57
Proteins expressed similarly in both treatments
015	Control/PEG6	Stress response, metabolic process	gi\|211906436	UDP-D-glucose pyrophosphorylase	Gossypium hirsutum	5	15%	213
024	Control/PEG6	Other functions	gi\|148912162	Cytosolic ascorbate peroxidase 1	Gossypium hirsutum	3	20%	221
027	Control/PEG6	Oxidation-redution process	gi\|449464176	PREDICTED: proteasome subunit beta type-3-A-like	Cucumis sativus	2	15%	175
028	Control/PEG6	Metabolic process	gi\|5669924	Soluble inorganic pyrophosphatase	Populus tremula x Populus tremuloides	1	7%	37
032	Control/PEG6	Stress response	gi\|226973430	Beta-thioglucoside glucohydrolase	Carica papaya	5	13%	278
044	Control/PEG6	Unclassified	gi\|412993224	ORF73	Bathycoccus prasinos	1	0%	36
045	Control/PEG6	Other functions	gi\|270313547	S-adenosylmethionine decarboxylase	Olea europaea	1	2%	47
051	Control/PEG6	Protein metabolism, Stress response	gi\|233955399	Calreticulin	Carica papaya	6	22%	323
052	Control/PEG6	Oxidation-redution process	gi\|193290660	Putative ketol-acid reductoisomerase	Capsicum annuum	3	10%	116
056	Control/PEG6	Unclassified	gi\|384249809	HI0933-like protein	Coccomyxa subellipsoidea	1	2%	58
060	Control/PEG6	Oxidation-redution process	gi\|449520553	PREDICTED: superoxide dismutase [Mn], mitochondrial-like isoform 1	Cucumis sativus	2	13%	204
061	Control/PEG6	Oxidation-redution process	gi\|33521626	Mn-superoxide dismutase	Lotus japonicus	1	8%	65
062	Control/PEG6	Unclassified	gi\|384253982	Hypothetical protein COCSUDRAFT_39121	Coccomyxa subellipsoidea C-169	1	1%	44
067	Control/PEG6	Unclassified	gi\|460412635	PREDICTED: 20 kDa chaperonin, chloroplastic-like	Solanum lycopersicum	3	12%	221
069	Control/PEG6	Stress response, signaling	gi\|2853219	Glutathione transferase	Carica papaya	2	10%	41
087	Control/PEG6	Others functions	gi\|55375985	14-3-3 family protein	Malus x domestica	6	33%	504
095	Control/PEG6	Stress response	gi\|357514981	Annexin-like protein	Medicago truncatula	2	5%	57
099	Control/PEG6	Strress response	gi\|356531939	PREDICTED: putative lactoylglutathione lyase-like	Glycine Max	3	9%	152
126	Control/PEG6	Metabolic process	gi\|5725356	Alpha-D-xylosidase	Tropaeolum majus	1	1%	37
003	Control/PEG6	Unclassified	gi\|357498189	DNA repair and recombination protein PIF1	Medicago truncatula	1	2%	40
007	Control/PEG6	Oxidation-redution process	gi\|12322163	Dormancy related protein, putative	Arabidopsis thaliana	1	3%	47

PEG: polyethylene glycol.

^aSpot numbers correspond to the numbers indicated in Figure 3.

^bMaturation treatments: Control - without PEG; and PEG6 - with 6% PEG.

^cFunctional protein classification using Blast2go (http://www.blast2go.com).

^dAccession number in the NCBI protein database.

^eNumber of unique peptide sequences identified by MASCOT.

^fPercentage of predicted protein sequence covered by MASCOT.

^gProbability-based MOWSE score from MASCOT software for the hit.

^hProtein relative abundance according to the individual spot (%) volume determined using Image Master Platinum v.7 software (GE Healthcare). Change in abundance levels by t-test, *(p ≤ 0.05), **(p ≤ 0.01), ns (not significant).