Skip to main content
. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: J Med Genet. 2010 Sep;47(9):595–600. doi: 10.1136/jmg.2009.075341

Figure 2. Co-immunoprecipitation analysis.

Figure 2

Upper panel: Neither the negative control 1 (lane 1) without FLAG-Stx11 nor the negative control 2 (lane 2) without EGFP-STXBP2 show unspecific binding to the IP antibody coupled agarose beads. EGFP-STXBP2 WT (lane 3) as well as both EGFP-STXBP2 polymorphic variants (lane 4/lane 5) co-immunoprecipitate with FLAG-Stx11, whereas EGFP-STXBP2 Gly541Ser (lane 6) and Glu132Ala (lane 7) show an impaired bingeing to FLAG-Stx11. The middle panel shows the corresponding input control of the EGFP-STXBP2 proteins. The lower panel presents the IP controls of each approach which reveal approximately equal amounts of FLAG-Stx11 after immunoprecipitation with the capture anti-FLAG-M2 murine IgG1 monoclonal antibody.