Figure 2.

Exacerbation of CCl₄-induced hepatocyte apoptosis by ethanol was attenuated in livers of ΔHepHIF1α^−/− mice. C57BL/6 wild-type (WT) and ΔHepHIF1α^−/− mice were allowed free access to diets with ethanol- (11% of kcal) or pair-fed controls for 4 days followed by CCl₄ injections, as described earlier. (A) Paraffin-embedded liver sections were deparaffinized and stained for M30/CK18 fragments, a marker for hepatocyte apoptosis. Images were acquired using a 20× objective. (B) Total numbers of M30⁺ cells were counted. Values with different alphabetical superscripts were significantly different from each other, P < 0.05 (n = 4–6).