Figure 3.

Exacerbation of CCl₄-induced p53 acetylation and PUMA expression by ethanol were attenuated in livers of ΔHepHIF1α^−/− mice. C57BL/6 wild-type (WT) and ΔHepHIF1α^−/− mice were allowed free access to diets with ethanol- (11% of kcal) or pair-fed controls for 4 days followed by CCl₄ injections, as described earlier. (A) Ac-p53^/K386, (B) Ac-p53^/K386 (green) and collagen 1 (red) costaining, or (C) PUMA. Images were acquired using a 40× objective. PUMA (green) was costained with the nuclear stain DAPI (blue). (D) Total number of PUMA⁺ cells was counted. Values with different alphabetical superscripts were significantly different from each other, P < 0.05 (n = 4–6).