Figure 6.

Exacerbation of CCl₄-induced Ly6c⁺ cell infiltration by ethanol were attenuated in livers of ΔHepHIF1α^−/− mice. C57BL/6 wild-type (WT) and ΔHepHIF1α^−/− mice were allowed free access to diets with ethanol- (11% of kcal) or pair-fed controls for 4 days followed by CCl₄ injections, as described earlier. (A) Frozen liver sections were used for Ly6c staining. Images were acquired using a 20× objective. (B) Total numbers of Ly6c⁺ cells were counted. Values with different alphabetical superscripts were significantly different from each other, P < 0.05 (n = 4–6). (C) Ly6C⁺ (green) and collagen 1 (red) were costained in frozen liver sections from C57BL/6 mice exposed to both ethanol and CCl₄. White arrows indicate collagen 1-stained fibers and blue arrows indicate Ly6C⁺-stained cells.