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. 2014 Jul 7;111(29):10696–10701. doi: 10.1073/pnas.1320833111

Fig. 4.

Fig. 4.

N-glycan processing is required for G-CSF–induced surface HECA-452 reactivity and MPO–EL expression. Flow cytometry analysis of BM cells treated with sialidase and cultured with G-CSF or G-CSF and DMJ: (A–C) HECA-452 reactivity and (D–F) MPO expression. (A and C) BMs in buffer alone (buffer treated) or treated with sialidase and cultured for 48 h (48-h recovery after sialidase). (A) Sialidase efficiency was confirmed before cell culturing (sialidase treated). (B and E) BM cells treated with sialidase and cultured for 48 h without G-CSF (48-h recovery after sialidase) or with G-CSF (48 h after sialidase + G-CSF). (B and E) BM cells treated with sialidase and cultured for 48 h with G-CSF and DMJ (48 h after sialidase + G-CSF + DMJ). (C) Cumulative results for expression of HECA-452 determinants on cells treated with sialidase and then cultured ± G-CSF and ± DMJ. Cells were isolated from different donors (n = 5), *P < 0.001. (F) Cumulative results for MPO surface expression on cells treated with sialidase and then cultured ± G-CSF and ± DMJ. Cells were isolated from multiple donors (n = 5), *P < 0.001.