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. 2014 Jul 8;111(29):10538–10543. doi: 10.1073/pnas.1402605111

Fig. 5.

Fig. 5.

The E3 ubiquitin ligase activity is required for the ER marker recruitment to the bacterial phagosome. (A) Images show the recruitment of the ER marker GFP-HDEL (green) to the LCVs in D. discoideum cells infected with the indicated Legionella strains (red). (Scale bars, 2 μm.) WT: L. pneumophila Philadelphia-1 wild-type strain Lp02; dotA: the type IV secretion system defective strain Lp03; ΔsidC-sdcA: the SidC and SdcA double deletion mutant of the Lp02 strain, ΔsidC-sdcA(pSidC); and ΔsidC-sdcA(pSidC C46A): ΔsidC-sdcA strain complemented with a plasmid expressing wild-type or C46A mutant SidC. (B) Percent of cells containing GFP-HDEL positive LCVs counted from three independent assays under the conditions infected with the indicated Legionella strains. *P < 0.01 compared with the infection with wild-type Legionella strain.