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. 2014 Jun 30;111(29):10514–10519. doi: 10.1073/pnas.1402216111

Fig. 4.

Fig. 4.

Conformal coating of rodent islets with 5% (wt/vol) PEG-VS/DTT and 0.8% MVG (PEG-ALG) does not impair in vitro and in vivo function in mice. (A) Phase-contrast image of rat islets conformally coated (CC) with PEG-ALG. (Scale bar, 200 μm.) Perifusion schematic (B) and results (C) of PEG-ALG CC rat islets compared with naked controls. Effect of ex vivo culture of rat naked islets (D) or CC (on day 2 after isolation) islets (E and F) on GSIR up to day 4 after isolation. (G and H) Effect of timing on GSIR of rat CC islets either day 1 or day 2 after isolation and assessed either 24 h or 48 h after encapsulation. Shown are mean ± SD with n = 3 for each value. LG = 2.2 mM and HG = 16.7 mM. *P < 0.05; ***P < 0.01. (I) Blood glucose of streptozotocin-induced diabetic C57BL/6 mice transplanted with either 700 or 1,500 IEQ of syngeneic naked control islets (n = 2) or PEG-ALG CC islets under the kidney capsule (n = 4). Nephrectomies were performed at day 52 and day 112 (arrows).