Table 2.
Data collection and refinement statistics
| hSLBP RBD pThr171-SL-3′hExo | dSLBP RPD-5EL245M/L253M | dSLBP RPD-5E | dSLBP RPD-WT | |
| Data collection | ||||
| Space group | P212121 | P4132 | P43212 | P43212 |
| Cell dimensions | ||||
| a, b, c, Å | 82.1, 91.5, 128.7 | 103.9, 103.9, 103.9 | 74.8, 74.8, 161.3 | 75.0, 75.0, 173.5 |
| α, β, γ, ° | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Resolution, Å | 50.0–2.3 (2.38–2.30) | 50–2.6 (2.64–2.60) | 50–2.9 (3.0–2.9) | 50–3.08 (3.15–3.10) |
| Rsym or Rmerge | 0.092 (0.48) | 0.099 (0.65) | 0.144 (0.47) | 0.099 (0.50) |
| I/σI | 9.5 (1.8) | 26.7 (2.6) | 13.6 (3.0) | 33.7 (2.6) |
| Completeness, % | 93.2 (84.5) | 99.8 (99.5) | 97.6 (81.6) | 93.4 (60.4) |
| Redundancy | 3.5 (3.5) | 23.8 (6.2) | 7.2 (5.5) | 12.3 (6.8) |
| Refinement | ||||
| Resolution, Å | 38.2–2.3 | 46.5–2.6 | 23.7–2.9 | 45.8–3.08 |
| No. reflections | 40,611 | 11,133 | 10,448 | 9,127 |
| Rwork/Rfree | 19.3/24.5 | 25.0/28.7 | 21.5/26.3 | 25.5/31.7 |
| No. atoms | 6,416 | 926 | 2,282 | 2,215 |
| Protein | 5,201 | 384 | 1,157 | 1,135 |
| RNA | 918 | 540 | 1,120 | 1,076 |
| Water/ion | 297 | 2 | 5 | 4 |
| B-factors | ||||
| Protein | 25.5 | 50.0 | 72.7 | 110.0 |
| RNA | 38.4 | 66.8 | 72.1 | 113.4 |
| Water/ion | 28.5 | 81.7 | 91.8 | 108.8 |
| Rms deviations | ||||
| Bond lengths, Å | 0.008 | 0.007 | 0.008 | 0.016 |
| Bond angles, ° | 1.1 | 1.2 | 1.2 | 1.4 |
Values in parentheses are for highest-resolution shell.