Fig. 2.

VEEV DI replicons express heterologous proteins more efficiently than those having standard design. (A) The schematic representation of VEEV replicons encoding GFP or Luc genes under control of the subgenomic promoters. (B) BHK-21 cells were infected with the indicated packaged replicons at an MOI of 20 inf.u/cell, and GFP fluorescence was evaluated by FACS analysis at 20 h postinfection. (C) Cells were infected with indicated replicons at the same MOI, harvested at 20 h postinfection, lysed in loading buffer, and samples were analyzed by SDS/PAGE, followed by Coomassie blue staining. Cm, noncytopathic VEEV capsid protein; nsP1Δ, a peptide, whose coding sequence contains a 51-nt CSE.