Fig. 4.
DI replicons demonstrate higher levels of heterologous protein synthesis, which correlates with the increase of SG RNA production. Infected cells were metabolically labeled with [35S]methionine at 7 h postinfection (Materials and Methods for details). Fragments of the same gel are presented. Radioactivity in the GFP- and Luc-containing bands was measured on Storm phosphorimager. The data were normalized to the radioactivity in the GFP and Luc bands detected in the VEErep/GFP and VEErep/Luc/GFP samples, respectively.