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Astrocytes that lack FGF signaling become reactive. Colabeling by immunofluorescence for GFAP+ protein and in situ hybridization for Fgfr1 or Erm RNA reveals that reactive astrocytes do not express Fgfr1 or Erm mRNA in the LOF mutant (n = 0 of 100 cells examined by confocal imaging). (Scale bar: 20 μm.)
