Skip to main content
. 2014 Jul 7;111(29):10755–10760. doi: 10.1073/pnas.1405190111

Fig. 3.

Fig. 3.

ML297- and baclofen-induced GIRK currents in hippocampal neurons. (A) Traces showing the effects of vehicle (0.1% DMSO), baclofen (100 µM), and ML297 (10 µM) on holding currents in neurons from wild-type (Upper) and Girk1−/− (Lower) mice. (B) Summary of peak current densities evoked by vehicle, baclofen, and ML297 in neurons from wild-type (WT) and Girk1−/− (Girk1) mice; a significant genotype × drug interaction was observed (F2,43 = 16.4; P < 0.001). ***P < 0.001 (within drug); ###P < 0.001 vs. ML297 (within genotype). (C) Acute desensitization of currents induced by baclofen (bac, 100 μM) and ML297 (10 μM), measured by comparing peak drug-induced currents with currents measured 20 s after drug application. Baclofen-induced currents showed modest acute desensitization (∼20%), whereas ML297-induced currents did not (t12 = 4.2; **P < 0.01). (D) Activation (t8 = 12.8; ***P < 0.001) and deactivation (t8 = 6.5; ***P < 0.001) kinetics of currents induced by baclofen and ML297 in hippocampal neurons from wild-type mice.