Fig. 2.
ST1120 increased the cleavage of a short fluorescent γ-secretase substrate peptide. (A and B) Effect of ST1120 on Vmax and Km of the recombinant γ-secretase complex derived from Sf9 cells calculated by fitting of the Hanes–Woolf plot (n = 4; mean ± SE; **P < 0.01). RFU, relative fluorescent units. (C) Coomassie Brilliant Blue staining of purified T4L-human PS1 protein (arrowhead) synthesized by an E. coli cell-free protein synthesis system. (D) Effect of 100 μM ST1120 on the cleavage of Nma-APP-Dnp by the purified PS1 protein. Relative velocities are shown (n = 4; data represent mean ± SE; **P < 0.01, ***P < 0.001, Student t test).