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Source and dilutions of primary antibodies used in immunohistochemistry (IHC) and immunoblotting (IB).
| Primary antibody | Species | Source (Catalog umber) | Dilution (IHC) | Dilution (IB) |
|---|---|---|---|---|
| AKR1B1 | Rabbit | Gift from Dr. Fortier | 1:100 | 1:2000 |
| hPGT | Rabbit | Gift from Dr. Fortier | 1:50 | 1:1000 |
| PGDH | Rabbit | Gift from Dr. Fortier | 1:100 | 1:100 |
| PGES | Rabbit | Cayman (160140) | 1:50 | 1:1000 |
| COX-2 | Goat | Santa Cruz (sc-1745) | 1:50 | 1:250 |
| RhoGDI | Goat | Santa Cruz (sc-360) | _ | 1:20000 |
