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. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: J Agric Food Chem. 2010 Oct 13;58(19):10330–10337. doi: 10.1021/jf101387v

Figure 1.

Figure 1

Gel electrophoresis of purified recombinant PlyCP26F and PlyCP39O accompanied by spot assays of C. perfringens on confluent plates. (A) SDS-PAGE is shown in a gel of 2.35 μg of nickel column-purified phage-derived lysin proteins. Lanes: MW, molecular weight markers; 1, Ply 26F;2, Ply 39O. Arrows indicate phage-derived lysins. (B) Spot assays were conducted by applying either bacterial lysates or the purified protein on lawns of C. perfringens as well as 10 μL of 10 mg/mL lysozyme and lysostaphin.