FIG. 6.

Expression of the p53 gene compared among the three cell lines in their differentiated and undifferentiated state. (A) Normalized data obtained by quantitative real-time PCR. ACTB, RPL32, TBP, and B2M were used for normalization. A, adipogenically differentiated; Chr, chondrogenically differentiated; Ost, osteogenically differentiated. (B) Representative melting curve obtained using primers specific for p53 as indicated in Table 1. (C) Representative PCR products obtained by quantitative PCR analyses. Left lanes, p53; right lanes, RPL32. Lane 1, NIH-3T3; lane 2, Flp-In-3T3; lane 3, MEF; lane 4, bone; lane M, marker.