Table 2.
Ct Values Obtained for Undifferentiated Flp-In-3T3, NIH-3T3, and MEF Cells Using Primers Specific for p53 As Stated in Table 1
| Sample | Ct value | Sample | Ct value | Sample | Ct value | Sample | Ct value |
|---|---|---|---|---|---|---|---|
| Flp | N/A | MEF | 27.7 | NIH | N/A | ||
| Flp | N/A | MEF | 28.6 | NIH | N/A | ||
| Flp | 35.8 | MEF | 27.4 | NIH | 34.7 | ||
| Fat | 28.9 | Flp-A | N/A | MEF-A | 30.4 | NIH-A | 32.1 |
| Fat | 29.2 | Flp-A | N/A | MEF-A | 30.7 | NIH-A | 32.3 |
| Fat | 28.9 | Flp-A | N/A | MEF-A | 30.9 | NIH-A | 32.6 |
| Cartilage | 29.6 | Flp-Chr | 38 | MEF-Chr | 27.9 | NIH-Chr | 30.3 |
| Cartilage | 31.6 | Flp-Chr | 35.3 | MEF-Chr | 28.1 | NIH-Chr | 30.5 |
| Cartilage | 31.3 | Flp-Chr | 35.4 | MEF-Chr | 28.4 | NIH-Chr | 31.2 |
| Bone | 32.4 | Flp-Ost | N/A | MEF-Ost | 29.4 | NIH-Ost | 33.6 |
| Bone | 31.7 | Flp-Ost | N/A | MEF-Ost | 31.1 | NIH-Ost | 33.9 |
| Bone | 32.4 | Flp-Ost | N/A | MEF-Ost | 30.4 | NIH-Ost | 32.2 |
The values are from a representative example from three independent runs.
Flp, Flp-In-3T3; MEF, mouse embryonic fibroblasts; NIH, NIH-3T3; A, adipogenically differentiated; Chr, chondrogenically differentiated; Ost, osteogenically differentiated.