FIG. 1.

Rapid Assessment of Migration and Proliferation (RAMP) arrays imaged by the flatbed fluorescent scanner are sensitive to differences in seeding density and matrix composition. Representative scans for phalloidin-stained Schwann cells cultured for 24 h in collagen (A) and collagen–Matrigel™ (B) were analyzed. (C) The average intensity increases significantly with increasing seeding density within both biomaterials for 250–1000 cells per μL densities (^#p<0.05, n=3, m=8). Intensity differences between the two model biomaterials can be detected at individual seeding densities from 63 to 500 cells per μL with collagen (black circles) exhibiting a significantly lower average intensity relative to the collagen–Matrigel biomaterial (gray squares) (⁺p<0.05, n=3, m=8). Error bars represent standard deviation.