Figure 5.

Role of PDE3-ICER feedback loop in the regulation of rat adult cardiomyocyte apoptosis. Rat adult cardiac myocytes were transduced with 100 multiplicities of infection of Ad-LacZ, Ad-PDE3A1, Ad-PKI, or Ad-AS-ICER, followed by the treatment with vehicle or Ang II (200 nmol/L) for 48 hours. A, Western blots showing the expression levels of ICER and Bcl2. B, Microscopic images of cardiomyocytes stained with TUNEL (left panels) and DAPI (4′,6′-diamido-2-phenylindole) (right panels). Arrows point to apoptotic cells showing positive nuclear TUNEL staining. C, Quantitative results of TUNEL experiments. Data are means±SEM (n=3). *P<0.vs Ang II+Ad-LacZ. Adapted from Ding et al.⁸⁷