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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Sep 19;33(11):2674–2681. doi: 10.1161/ATVBAHA.113.302137

Figure 4. Binding of EPCR variants to fluorescently-labelled APC (Fl-APC) and FITC-JRK 1535 (anti-EPCR mAb).

Figure 4

HEK293 cells stably expressing wild-type human EPCR or EPCR variants (Arg96Cys and Val170Leu) were incubated at room temperature with 100 nM of either Fl-APC (solid line) or FITC-labelled JRK 1535, a monoclonal antibody against human EPCR (dotted line). Binding of these ligands to the cells was analyzed by flow cytometry. Top panel, wildtype EPCR; middle panel, EPCR Arg96Cys; bottom panel, EPCR Val170Leu.