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PolySia is expressed predominantly on myeloid cells in murine bone marrow. Freshly isolated murine BM cells were either exposed to endoN (top row) or mock-treated (bottom row), double-stained with GFP-endoN and an isotype control or various antibodies to hematopoietic lineage markers (CD11b, Ly6 G/C, CD3e, CD45R/B220 and TER-119), and analyzed by flow cytometry. In panels showing staining with Ly6 G/C, neutrophils (N) and monocytes/macrophages (M) are denoted by arrows.
