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. 2014 May 1;25(8):1662–1668. doi: 10.1681/ASN.2013040425

Figure 3.

Figure 3.

Long-term delivery of suPAR in WT mice. Seven-day sustained delivery of recombinant mouse suPAR (200 µg) by mini-osmotic pumps in normal WT mice (C57BL6/J). (A) Albuminuria (ELISA) evaluated after 7 days of mouse suPAR delivery; absence of any induction of albuminuria between NaCl-treated control (CTL) group versus the suPAR-delivered group (n=8–10 mice per group). (B and C) Western blot detection of uPAR in lysates of kidney cortex 7 days after mini-pump installation showing a massive accumulation of suPAR in the kidney (n=4 per group). The arrows indicate suPAR, and the double band aspect represents the endogenous glycosylation heterogeneity of mouse uPAR. (D) Immunohistochemical detection of suPAR deposited in the glomeruli of mouse kidneys 7 days after mini-pump installation. Deposits are found essentially on glomerular structures (magnification ×400). Overall, the subcutaneous delivery of 200 µg of recombinant suPAR over a week failed to induce any albuminuria in normal mice despite suPAR deposits/accumulation on glomerular structures. All results are expressed as mean±SD (n=8 per group); *P<0.05 (one-way ANOVA).