Figure 4.

HIV-1 Tat protein is associated with LRs in human podocytes isolated from the urine of children with HIVAN. LRs were isolated from a cultured podocyte cell line (P2) infected with adenovirus carrying (A) FLAG-tagged Tat (Ad-Tat-FLAG) or (B) GFP (Ad-GFP) or (C) primary podocytes infected with Ad-Tat-FLAG by sucrose gradient–based flotation assay. Twelve fractions of the gradient were run by SDS-PAGE to show the distribution of DRM and non-DRM fractions. FLAG-tagged Tat, GFP, and the LR marker Caveolin-1 (Cav-1) were analyzed by Western blotting.