Table 1.

Effects of various suPAR types on podocyte integrin activation and kidney filter function

suPAR Type	Source	Accession	Recipient	Route	Dose (μg)	Time	Glomerular β3 Integrin Activity	Kidney Phenotype	Reference
Full length, isoform 1, mouse	Drosophila S2 cells	NM_01111	B6 or 129 mice	Intravenous	Up to 100	24 h	Not studied	No proteinuria, no podocyte FP effacement	2
Full length, isoform 1, Fc chimera, mouse	Mouse NS0 cells	Q545X5	B6 or 129 mice	Intravenous	Up to 100	24 h	Not studied	No proteinuria, no podocyte FP effacement	2
Full length, isoform 1, mouse	Drosophila S2 cells	NM_01111	B6 mice	Osmotic pump	200	1 wk	Not studied	No proteinuria, no FP effacement	2
Full length, isoform 1, Fc chimera mouse	Mouse NS0 cells	Q545X5	uPAR KO mice 129/B6	Intravenous	20	24 h	Increased	Proteinuria	1
Endogenous	LPS induced	—	B6 mice	Endogenous	N/A	24 h	Increased	Serum and urinary suPAR increased, proteinuria, podocyte FP effacement	1
Endogenous	LPS induced	—	uPAR null kidney engrafted into B6 mice	Host suPAR circulating into the engrafted uPAR null kidney	N/A	24 h	Increased	suPAR deposits into uPAR null kidney, podocyte FP effacement	1
Secreted, isoform 2	Plasmid DNA	BC010309	B6 mice	Intradermal electroporation	80 DNA weekly	1 mo	Increased	FSGS-like changes, proteinuria	1, 11

S2, Schneider 2; KO, knockout; FP, foot process.