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. 2014 Aug 1;16(4):253–265. doi: 10.1089/cell.2013.0058

FIG. 1.

FIG. 1.

Treatment of oxamflatin resulted in the histone hyperacetylation status of donor nucleus of porcine SCNT embryos. (A) Relative HDAC activities between nontreated (C-NT) and treated (T-NT) porcine SCNT embryos. Embryos were collected 15 h after activation. HDAC activities of embryos were measured using a short peptide substrate, BOC-(Ac) Lys-AMC. (B and C) Quantification of AcH3K9 and AcH4K5 mean fluorescence signals intensities of porcine SCNT embryos between C-NT and T-NT groups, respectively. Labeling intensity was expressed relative to that of the C-NT embryos. (D) The relative expression levels of histone deacetylation-related genes in MII oocytes and PFF cells. (E) The relative expression levels of HDAC1, -2, and -3 in MII, C-NT, and T-NT groups. Relative expression level of mRNA was calculated relative to β-actin. MII, oocytes in metaphase II stage; T-NT, SCNT embryos treated with 1 μM oxamflatin for 15 h postactivation; C-NT, nontreated SCNT embryos. Data are presented as mean±standard deviation (SD). (*) p<0.05; (**) p<0.01).