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. 2014 Aug 1;16(4):253–265. doi: 10.1089/cell.2013.0058

FIG. 4.

FIG. 4.

The effects of oxamflatin on the expression level of pluripotency-related genes and the locus-specific methylation status of satellite DNA sequences and POU5F1 promoter in porcine SCNT blastocysts. (A) Relative expression levels of POU1F, SOX2, and KLF4 in day-7 blastocysts derived from porcine SCNT embryos not treated (C-NT) or treated (T-NT) with 1 μM oxamflatin for 15 h postactivation. The relative expression level of mRNA was normalized to β-actin. Data are presented as mean±standard deviation (SD). (**) p<0.01. (B and C) Methylation profile analysis of porcine satellite sequences (GenBank accession no. Z75640.1) and POU5F1 promoter (GenBank accession no. CT737281.12) in day-7 porcine blastocysts between C-NT and T-NT groups, respectively. Open and filled circles, unmethylated and methylated CpG sites, respectively; horizontal lines of circles, one separated clone. Some CpG sites are absent from the satellite sequence in some clones due to mutations in the particular copies of the satellite sequences. Numbers at the bottom of the chart indicate the proportion of methylated CpG sites relative to the whole CpG sites counted.