Table 2. Gene Ontology categories in three different transcriptomes from samples of the Ixodes scapularis female synganglion: GO terms searching success, similarities and differences.
BioProcess Level 2 (GO term) | % Sample Il-1 | % Sample Il-2 | % Sample 454 | |
Metabolic and cellular processes | 60.3 | 69.7 | 50.4 | |
Biological regulation | 8.9 | 11.6 | 9.2 | |
Signaling | 4.0 | 4.1 | 4.5 | |
Response to stimulus | 5.7 | 2.0 | 6.7 | |
Developmental processes | 1.9 | 1.3 | 3.7* | |
Immunity | 0.4 | 0.3 | 0.8 | |
Locomotion | 0.5 | 0.2 | 0.8 | |
Growth | 0.4 | 0.1 | 0.7 | |
Reproduction | 0.5 | 0.1 | 0.9 | |
Totals (% all categories) | 82.5 | 89.5 | 73.2 | |
Search success: total transcripts/(% all transcripts) | 8,086 (19.6%) | 8,445 (27.5%) | 9,574 (46.4%) | |
BioProcess Level 2 (GO term) | Metabolic and cellular processes | 50.8 | 46.3 | 46.5 |
Biological regulation | 5.3 | 5.9 | 7.3 | |
Oxidation-reduction | 4.1 | 3.3 | 3.1 | |
Signaling | 0.8 | 5.2* | 1.3 | |
Cellular response to stimulus | 3.0 | 0.8* | 3.3 | |
Response to external stimuli | 1.4 | 1.0 | 3.0 | |
Cellular developmental processes | 0.5 | 0.7 | 1.3* | |
Immunity | 0.2 | 0.3 | 0.3 | |
Hormonal metabolic processes | 0.6 | 2.4* | 0.1 | |
Growth | 0.0 | 0.1 | 0.2 | |
Reproduction | 0.5 | 0.3 | 0.9 | |
Totals (% all categories) | 67.2 | 66.3 | 67.3 | |
Search success: total transcripts/(% all transcripts) | 11,740 (28.5%) | 23,573 (76.4%) | 14,249 (69.1%) | |
Molecular Level 3 (GO term) | Hydrolase activity | 13.1 | 12.7 | 11.8 |
Transferase activity | 12.6 | 11.9 | 9.5 | |
Ion binding | 11.7 | 10.8 | 8.3 | |
Nucleotide binding | 8.8 | 9.2 | 10.7 | |
Nucleoside binding | 0.1 | 6.8* | 6.5 | |
Nucleic acid binding | 9.4 | 10.3 | 7.8 | |
Tetrapyrrole binding | 1.2 | 0.8 | 1.0 | |
Protein binding | 8.4* | 10.0* | 9.1 | |
Carbohydrate binding | 0.5 | 0.5 | 0.6 | |
Carboxylic acid binding | 0.3 | 0.2 | 0.2 | |
Lipid binding | 0.5 | 0.4 | 2.0 | |
Oxireductases activity | 6.5 | 4.8 | 8.0 | |
Signal transducer activity | 3.4 | 4.4 | 2.9 | |
Neurotransmitter binding | --- | 0.1 | 0.1 | |
Kinase regulatory activity | --- | 0.1 | 0.2 | |
Transmembrane transporter activity | 3.0 | 2.9 | 2.8 | |
Nucleoside-triphosphatase activity | 1.0 | 1.0 | 0.2 | |
Enzyme inhibitor activity | 0.7 | 0.5 | 0.8 | |
Enzyme activator activity | 0.6 | 0.5 | 0.2 | |
Totals (selected gene categories) | 81.8 | 65.5 | 83.3 | |
Search success: total transcipts/(% all transcripts) | 8,160 (19.8%) | 17,660 (57.3%) | 2,629 (12.74%) |
* Major difference.