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. 2014 Jun 5;3(8):888–898. doi: 10.5966/sctm.2013-0213

Figure 7.

Figure 7.

Neurotoxicity induced by 6-OHDA in Atoh1-induced dopaminergic (DA) neurons. (A): Atoh1-induced DA neurons derived from Atoh1-iPSCs at differentiation day 36 were treated with 6-OHDA for 15 minutes. Bright-field microscope images show the morphological signs of neuron death at 24 hours after treatment. Scale bar = 100 μm. (B): Cell death was quantified by LDH cytotoxicity assay. The data represent means ± SEM (n = 3). ∗, p < .01 compared with control. Abbreviations: LDH, lactate dehydrogenase; 6-OHDA, 6-hydroxydopamine.