TABLE 1:
S. cerevisiae genes whose deletion led to a strong increase in Aβ42-GFP–associated fluorescence, together with the respective localization of fluorescence.
| Open reading frame/gene name | Aβ42-GFP localization pattern | Respiratory deficiency | Human orthologue |
|---|---|---|---|
| Chromatin remodeling/histone exchange | |||
| CHD1 | Cytosolic | No | CHD2 |
| HIR1 | Single small punctate | No | HIRA |
| HTA2 | Single small punctate | No | H2AFX |
| SWC5 | Single small punctate | No | CFDP1 |
| SWR1 | Multiple puncta | No | |
| VPS71 | Multiple small puncta | No | |
| VPS72 | Cytosolic with single large punctate | No | |
| YDL041W | Multiple small puncta | No | |
| Lipid metabolism/transport | |||
| CHO2 | Cytosolic with one or two small puncta | No | |
| DET1 | Cytosolic | No | |
| INO2 | ER-associated | No | |
| INO4 | Punctate | No | |
| IPK1 | Cytosolic with single large punctate | No | |
| OPI3 | ER-associated | No | PEMT |
| PDX3 | Cytosolic with punctate and nuclear | Yes | PNPO |
| PSD1 | ER-associated | No | PISD |
| SCS2 | Single large punctate | No | VAPA |
| UME6 | Single small punctate | No | |
| YER119C-A | Single small punctate | No | |
| Mitochondrial functions | |||
| ACO1 | Cytosolic with one or two small puncta | Yes | ACO2 |
| ACO2 | Cytosolic | No | |
| AIM4 | Single small punctate | Yes | |
| ATP11 | Cytosolic with one or two puncta | Yes | ATPAF1 |
| CBP3 | Cytosolic with puncta | Yes | UQCC |
| CIT1 | Cytosolic with one or two small puncta | Yes | |
| CIT3 | Cytosolic with one or two small puncta | No | |
| COX16 | Cytosolic | Yes | |
| COX20 | Cytosolic | Yes | |
| CYM1 | Single small punctate | No | PITRM1 |
| FUM1 | Cytosolic with puncta | Yes | FH |
| HAP2 | Cytosolic | Yes | NFYA |
| HAP3 | Single small punctate | Yes | NFYB |
| IDH1 | Cytosolic with small puncta | Yes | IDH3B |
| IDH2 | Cytosolic with small puncta | Yes | IDH3A |
| IDP1 | Cytosolic with small puncta | No | IDH1 |
| KGD1 | Cytosolic | Yes | OGDH |
| KGD2 | Cytosolic | Yes | DLST |
| LPD1 | Cytosolic | Yes | DLD |
| LSC1 | Cytosolic | Yes | SUCLG1 |
| LSC2 | Cytosolic | No | |
| MDH1 | Cytosolic | No | MDH2 |
| MIC14 | Cytosolic | No | |
| MRPL35 | Cytosolic | Yes | MRPL35 |
| MRPL7 | Cytosolic with small puncta | Yes | |
| PET112 | Single small punctate | Yes | PET112L |
| PET117 | Cytosolic | Yes | |
| PYC1 | Cytosolic with small puncta | No | PC |
| PYC2 | Cytosolic | No | PC |
| RIM1 | Cytosolic | Yes | |
| RRG8 | Large punctate and nuclear-diffused | Yes | |
| RSM18 | Single small punctate | Yes | |
| RSM7 | Cytosolic | Yes | |
| SDH1 | Cytosolic | No | SDHA |
| SDH2 | Cytosolic | Yes | SDHB |
| SDH4 | Cytosolic with puncta | Yes | SDHD |
| STF2 | Cytosolic with single large punctate | No | |
| TUF1 | Single small punctate | Yes | TUFM |
| YDR230W | Cytosolic | Yes | |
| Gene expression/regulation | |||
| CTK1 | One or two small puncta | No | |
| DEG1 | Single large punctate | No | PUS3 |
| ELC1 | Single small punctate | No | |
| GAT1 | Single small punctate | No | |
| GDT1 | Single small punctate | Yes | TMEM165 |
| HFI1 | Cytosolic | Yes | |
| LSM7 | Multiple small puncta | Yes | LSM7 |
| MED1 | Cytosolic with one or two small puncta | No | MED1 |
| MOT2 | Single small punctate | Yes | |
| NCL1 | Single small punctate | No | NSUN2 |
| SNT309 | Cytosolic with one or two small puncta | Yes | |
| SRB8 | One or two small puncta | Yes | MED12L |
| SSN2 | Cytosolic with single large punctate | No | MED13L |
| SSN3 | Multiple small puncta | Yes | CDK8 |
| SYC1 | Single small punctate | No | |
| TIF4631 | Multiple small puncta | Yes | EIF4G1 |
| Mitotic cell cycle | |||
| CTS1 | Cytosolic | No | |
| DCC1 | Single small punctate | Yes | DSCC1 |
| POG1 | Single small punctate | No | |
| SWI4 | Cytosolic with single small punctate | No | |
| Methionine metabolism | |||
| MET16 | Single small punctate | No | |
| MET8 | Single small punctate | No | |
| MXR1 | Single small punctate | No | MSRA |
| Purine metabolism | |||
| ADE12 | Single small punctate | No | ADSSL1 |
| ADK1 | Single small punctate | No | AK2 |
| Spindle pole body | |||
| BFA1 | Single small punctate | No | |
| BIM1 | Single small punctate | No | MAPRE1 |
| Ubiquitin/proteasome | |||
| SAN1 | Cytosolic | No | |
| SHP1 | Cytosolic with puncta | Yes | NSFL1C |
| UBR1 | Cytosol with large punctate | No | UBR1 |
| Bud-site selection | |||
| BUD23 | Cytosolic with punctate | Yes | WBSCR22 |
| BUD31 | Cytosolic with puncta | Yes | BUD31 |
| MAP kinase activity | |||
| PBS2 | Single small punctate | No | MAP2K4 |
| SLG1 | Single small punctate | No | |
| SOK1 | Multiple small puncta | No | |
| Others/unknown | |||
| APJ1 | Single small punctate | No | |
| ASM4 | Cytosolic with large punctate | No | |
| EMI2 | Cytosolic with small puncta | No | |
| GTT3 | Single small punctate | No | |
| ICE2 | ER-associated | No | |
| ICY2 | Single small punctate | No | |
| PAU11 | Cytosolic | No | |
| PHM6 | Cytosolic puncta | No | |
| RAD61 | Cytosolic punctate | No | |
| RIB1 | Multiple puncta | Yes | |
| RKM4 | Single small punctate | No | |
| SNX41 | Cytosolic with one or two small puncta | No | |
| YDL242W | Cytosolic | No | |
| YDR015C | Cytosolic | No | |
| YEL008W | Single small punctate | No | |
| YIM2 | Cytosolic | No | |
| YOR364W | Single small Punctate | No | |