FIGURE 3.

Disruption of feedback inhibition blocks CTP synthase filament formation independent of catalytic activity. (A) Representative images of yeast strains expressing wild-type (WT), CTP-binding mutant, or CTP-binding and catalytic double mutant Ura7p-GFP. Average percentage and SEM of cells with Ura7p-GFP structures are indicated below each image. (B) Percentages of foci (<0.75 μm) and filaments (≥0.75 μm) for Ura7p-GFP structures are graphed for WT Ura7p-GFP and each mutant for comparison.