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Mutations in the ATP-binding site of CTP synthase increase filament formation. (A) Representative images of yeast strains expressing wild-type (WT), E146A, and D70A Ura7p-GFP. Average percentage and SEM of cells with Ura7p-GFP structures are indicated below each image. (B) Percentages of foci (<0.75 μm) and filaments (≥0.75 μm) for Ura7p-GFP structures are graphed for WT Ura7p-GFP and each mutant for comparison.
