Fig. 3.

Picomolar Aβ₄₂ peptides enhance spontaneous astrocyte calcium transients. A) Frequency of spontaneous calcium transients. 200 pM Aβ₄₂ (freshly prepared or aged oligomerized) were applied at the beginning of the second (20 min) imaging block (indicated by arrow). Vehicle n = 16 (4554 cells), fresh Aβ₄₂ 200 pM n = 18 (5241 cells), aged Aβ₄₂ 200 pM n = 17 (4798 cells). B) Amplitude of spontaneous calcium transients corresponding to A. C) Frequency of spontaneous calcium transients. 200 pM Aβ peptides (freshly prepared, Aβ₄₂, or Aβ₄₀) were applied at the beginning of the second (20 min) imaging block (arrow). Vehicle n = 16 (4554 cells), Aβ₄₂ 200 pM n = 18 (5241 cells), Aβ₄₀ 200 pM n = 6 (1723 cells). D) Amplitude of spontaneous calcium transients corresponding to C. E) Frequency of spontaneous calcium transients. 200 pM Aβ₄₂ (freshly prepared) and/or 6E10 antibodies were applied at the beginning of the second (20 min) imaging block (arrow). Vehicle n = 16 (4554 cells), Aβ₄₂ 200 pM n = 18 (5241 cells), 6E10 n = 4 (1209 cells), Aβ₄₂ 200 pM + 6E10 n = 8 (2395 cells). F) Amplitude of spontaneous calcium transients corresponding to E. G) Summary of frequency data at the 30 and 40 min imaging blocks. H) Summary of amplitude data at the 30 and 40 min imaging blocks. Data presented as means ± SEM, normalized as fold changes from the baseline control block. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to vehicle control by ANOVA.