Figure 8. MMP and TIMP expression in whole liver and primary hepatocytes from CCl₄ induced liver injury.

Quantitative PCR of MMP and TIMP expression in whole liver and isolated primary hepatocytes. Cirrhosis was induced in a mouse model of liver injury (C57bl/6) with CCl₄. RNA extracted from primary hepatocytes as well as whole liver were assessed at commencement of injury and weeks 1, 2 and 4. The expression of MMP-2 (Panel A), MMP-9 (Panel B), MMP-13 (Panel C), MMP-14 (Panel D), TIMP-1 (Panel E) and TIMP-2 (Panel F) was assessed by quantitative PCR (n = 4 per group, data expressed as mean and SEM. *p<0.05 and **p<0.01 relative to untreated).