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. 2014 Jul 14;10:107–113. doi: 10.4137/EBO.S13739

Figure 2.

Figure 2

miR-7 reduces PAX6 protein expression in human cells without inducing PAX6 mRNA decay. (A) Western blot analysis of HeLa cells transfected with hsa-miR-7 (lane 1) or control non-coding miR-NC miRNA (lane 3) using antibodies against PAX6 (top) and β-actin (bottom). A protein marker (lane 2) confirmed the correct size of PAX6 (48 kDa) and β-actin (42 kDa) proteins. (B) RT-qPCR analysis of PAX6 mRNA isolated from HeLa cells transfected with miRNA precursors corresponding to hsa-miR-7 (miR-7) and a negative control (miR-NC). PAX6 message levels were normalized relative to GAPDH and are presented as mean + SD.