Table 2.

Oligonucleotides used for generating firefly luciferase constructs.

TARGET SITES	OLIGONUCLEOTIDES (5′→3′)
m8#1	WT	SE	AGCTTTCTGAGGATTTCTAGGGAAGACAAATACTTACATTTTGACATAAAACAAATT
		AS	CTAGAATTTGTTTTATGTCAAAATGTAAGTATTTGTCTTCCCTAGAAATCCTCAGAA
	Mut	SE	AGCTTTCTGAGGATTTCTAGGAAGGGCAAATACTTACATTTTGACATAAAACAAATT
		AS	CTAGAATTTGTTTTATGTCAAAATGTAAGTATTTGCCCTTCCTAGAAATCCTCAGAA
m8#2	WT	SE	AGCTAGTGAAGTTTGCAGAGGAAGACTTATCTGTATTGACTTATATGTTGCACAGA
		AS	CTAGTCTGTGCAACATATAAGTCAATACAGATAAGTCTTCCTCTGCAAACTTCACT
	Mut	SE	AGCTAGTGAAGTTTGCAGAGAAGGGCTTATCTGTATTGACTTATATGTTGCACAGA
		AS	CTAGTCTGTGCAACATATAAGTCAATACAGATAAGCCCTTCTCTGCAAACTTCACT
A1# 3	WT	SE	AGCTCAAATAATCTCCATCCTGGAAGATGGTGTCAAAACATCCCTGCAGATACCCCA
		AS	CTAGTGGGGTATCTGCAGGGATGTTTTGACACCATCTTCCAGGATGGAGATTATTTG
	Mut	SE	AGCTCAAATAATCTCCATCCTGAAGGGTGGTGTCAAAACATCCCTGCAGATACCCCA
		AS	CTAGTGGGGTATCTGCAGGGATGTTTTGACACCACCCTTCAGGATGGAGATTATTTG

Notes: List of sense (SE) and antisense (AS) oligonucleotides used for generating firefly luciferase constructs containing WT and Mut miR-7 7mer target sites (m8#1, m8#2, and A1#3). Italic, bold, and underlined nucleotides represent restriction recognition-, miR-7 target- and Mut sites, respectively.