Fig. 7.

A miR-1289 mimic suppresses HKα 3′ UTR-Luc activity and HKα protein expression. A: AGS cells were transfected with the HKα 3′ UTR-Luc construct and then transfected with a synthetic 23-nucleotide oligoribonucleotide mimetic of miR-1289. Values are means ± SE (n = 3). B: human gastric corpus biopsies were transfected with the miR-1289 mimetic oligonucleotide, and biopsy lysates were analyzed by Western blot using monoclonal antibody HK 12.18 specific for HKα protein. β-Actin signal served as a gel loading control. The immunoblot image shown is representative of 3 replicate experiments. C: Image J quantitation of HKα-specific gastric biopsy lysate immunoblots as described in B (n = 3). AUC, area under the curve.